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KMID : 0362420170550040361
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Journal of Korean Academy of Prosthodontics
2017 Volume.55 No. 4 p.361 ~ p.371
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Regulation of human gingival fibroblast gene expression on microgrooves: A DNA microarray study
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Lee Kyung-Ho
Lee Sung-Bok
Ahn Su-Jin
Park Soo-Jeong
Lee Suk-Won
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Abstract
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PURPOSE. We aimed to investigate the gene expression of human gingival fibroblasts on microgroove surface using DNA microarray.
MATERIALS AND METHODS. Microgrooves were applied on grade II titanium discs to have 0/0 ㎛. (NE0, control group), 60/10 ㎛. (E60/10, experimental group) of respective width/depth by photolithography. The entire surface of the microgrooved Ti substrata was further acid etched and used as the two experimental groups in this study. Human gingival fibroblasts were cultured in the experimental group and the control group, and total RNA was extracted. The oligonucleotide microarray was performed to confirm the changes of various gene expression levels between experimental group and control group. Changes of gene expression level were determined at the pathway level by mapping the expression results of DNA chips, using the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis.
RESULTS. Gene expression levels on E60/10 and NE0 were analyzed, there were 123 genes showing significant differences in expression more than 1.5 times on E60/10 microgrooved surface compared to NE0 surface, and 19 genes showing significant differences in expression more than 2 times. The KEGG pathway analysis confirmed the changes in gene expression levels under experimental conditions. Cell signaling, proliferation, and activity among the various gene expression results were identified.
CONCLUSION. Microgrooved surfaces induce gene expression changes and related cell signaling. According to the results of this study, microgrooves can be used as the surface of various biomaterials which need to improve cell activity through gene expression changes and activation of cell signaling.
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KEYWORD
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Titanium, Microgrooves, Human gingival fibroblasts, Gene expression changes
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